The western blot, also known as protein immunoblot antibody, is widely utilized as an analytical technique for the detection of specific proteins in a sample of tissue extract. To know about antibody company, monoclonal antibodies you can search the browser.
This technique, to be short, is to stain the protein treated by gel electrophoresis with specific antibodies and then to transfer the stained protein onto the membrane, which hence enables the researchers to get the information expressed by the specific protein in the analyzed cell or tissue.
The name western blot was given because of its similarity with the procedure of Southern blot, a technique for DNA detection developed earlier. It plays an important rule in the experiments in the fields of immunogenetics, molecular biology, and other molecular biology disciplines.
Steps are taken during the experiment:
Tissue Preparation: Samples are taken from whole tissue or from cell culture
Gel electrophoresis: Proteins of the sample are separated using gel electrophoresis which is used to resolve of the problem of cross-reactivity of antibodies.
Transfer: Separated proteins are moved from within the gel onto a membrane.
Thousands of antibodies can be utilized in the various western blotting. One of them (both monoclonal and polyclonal) is the Anti-CDC25C monoclonal antibody, which is also known as Mouse monoclonal antibody Anti-Human CDC25C. This antibody is generated from a mouse immunized with CDC25C recombinant protein.